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cd45 antibody, anti-human  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd45 antibody, anti-human
    Cd45 Antibody, Anti Human, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 359 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd45+antibody/CD45+Antibody%2C+anti-human/custom%40130-108-020%4042727576
    Average 96 stars, based on 359 article reviews
    cd45 antibody, anti-human - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Staining:

    Article Title: Integrative Multiomic Classification Reveals Distinct Origins and Evolutionary Trajectories of Thymic Epithelial Tumors
    Article Snippet: Then, red blood cell lysis was done by treating 2 mL ACK lysis buffer (Gibco, #A1049201) for 5 minutes at room temperature, followed by PBS washing. .. Leukocytes were stained with CD45 antibody (Miltenyi Biotec, #130-045-801, RRID: AB_2783001) and depleted with autoMACS Pro Separator (Miltenyi Biotec). .. After washing, a single-cell RNA-seq library was generated using Chromium Single Cell 3′ Library and Gel Bead Kit v2 (10x Genomics).

    Article Title: Iron diminishes immunosuppressive macrophages and enhances anti-PD-1 immunotherapy in breast cancer models.
    Article Snippet: Following Ficoll separation, dead cells were removed by using dead cell removal kit (Cat#130–090–101, Miltenyi Biotec) per manufacturer’s instruction. .. The live cell fraction was blocked by incubation with TruStain FcX in 50 μL cell staining buffer for 20 min on ice, then incubated with magnetically labeled CD45 antibody (Cat#130–052–301, Miltenyi Biotec) and passed through a LS magnetic column (Cat#130–042– 401, Miltenyi Biotec). ..

    Article Title: Integrative Multi-omic Classification Reveals Distinct Origins and Evolutionary Trajectories of Thymic Epithelial Tumors
    Article Snippet: Then, RBC lysis was done by treating 2ml ACK lysis buffer (Gibco #A1049201) for 5 minutes at room temperature, followed by PBS washing. .. Leukocytes were stained with CD45 antibody (Miltenyi Biotec #130-045-801, RRID: AB_2783001) and depleted with autoMACS Pro Separator (Miltenyi Biotec). .. After washing, a single-cell RNA sequencing library was generated using Chromium Single Cell 3’ Library & Gel Bead Kit v2 (10x Genomics).

    Article Title: Iron diminishes immunosuppressive macrophages and enhances anti-PD-1 immunotherapy in breast cancer models
    Article Snippet: Following Ficoll separation, dead cells were removed by using dead cell removal kit (Cat#130–090–101, Miltenyi Biotec) per manufacturer’s instruction. .. The live cell fraction was blocked by incubation with TruStain FcX in 50 μL cell staining buffer for 20 min on ice, then incubated with magnetically labeled CD45 antibody (Cat#130–052–301, Miltenyi Biotec) and passed through a LS magnetic column (Cat#130–042–401, Miltenyi Biotec). ..

    other:

    Article Title: Protocol for isolating stromal cells from lymphoid tissue for performing scRNA-seq
    Article Snippet: CD45 antibody, anti-mouse, Biotin (Dilutions in 1:50) , Miltenyi Biotec , Cat# 130-124-209, RRID: AB_2819580.

    Article Title: Protocol for 3D-guided sectioning and deep cell phenotyping via light sheet imaging and 2D spatial multiplexing
    Article Snippet: CD45 antibody, anti-human, Vio R667, REAfinity , Miltenyi Biotec B.V. & Co. KG , Cat# 130-128-742 RRID: AB_2905022.

    Article Title: Protocol for 3D-guided sectioning and deep cell phenotyping via light sheet imaging and 2D spatial multiplexing
    Article Snippet: CD45 antibody, anti-human, FITC, REAfinity , Miltenyi Biotec B.V. & Co. KG , Cat# 130-110-631 RRID: AB_2658237.

    Incubation:

    Article Title: Iron diminishes immunosuppressive macrophages and enhances anti-PD-1 immunotherapy in breast cancer models.
    Article Snippet: Following Ficoll separation, dead cells were removed by using dead cell removal kit (Cat#130–090–101, Miltenyi Biotec) per manufacturer’s instruction. .. The live cell fraction was blocked by incubation with TruStain FcX in 50 μL cell staining buffer for 20 min on ice, then incubated with magnetically labeled CD45 antibody (Cat#130–052–301, Miltenyi Biotec) and passed through a LS magnetic column (Cat#130–042– 401, Miltenyi Biotec). ..

    Article Title: Iron diminishes immunosuppressive macrophages and enhances anti-PD-1 immunotherapy in breast cancer models
    Article Snippet: Following Ficoll separation, dead cells were removed by using dead cell removal kit (Cat#130–090–101, Miltenyi Biotec) per manufacturer’s instruction. .. The live cell fraction was blocked by incubation with TruStain FcX in 50 μL cell staining buffer for 20 min on ice, then incubated with magnetically labeled CD45 antibody (Cat#130–052–301, Miltenyi Biotec) and passed through a LS magnetic column (Cat#130–042–401, Miltenyi Biotec). ..

    Labeling:

    Article Title: Iron diminishes immunosuppressive macrophages and enhances anti-PD-1 immunotherapy in breast cancer models.
    Article Snippet: Following Ficoll separation, dead cells were removed by using dead cell removal kit (Cat#130–090–101, Miltenyi Biotec) per manufacturer’s instruction. .. The live cell fraction was blocked by incubation with TruStain FcX in 50 μL cell staining buffer for 20 min on ice, then incubated with magnetically labeled CD45 antibody (Cat#130–052–301, Miltenyi Biotec) and passed through a LS magnetic column (Cat#130–042– 401, Miltenyi Biotec). ..

    Article Title: Iron diminishes immunosuppressive macrophages and enhances anti-PD-1 immunotherapy in breast cancer models
    Article Snippet: Following Ficoll separation, dead cells were removed by using dead cell removal kit (Cat#130–090–101, Miltenyi Biotec) per manufacturer’s instruction. .. The live cell fraction was blocked by incubation with TruStain FcX in 50 μL cell staining buffer for 20 min on ice, then incubated with magnetically labeled CD45 antibody (Cat#130–052–301, Miltenyi Biotec) and passed through a LS magnetic column (Cat#130–042–401, Miltenyi Biotec). ..



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